![](https://parts.igem.org/images/partbypart/icon_coding.png)
Coding
HF Phusion
Part:BBa_K3271027:Design
Designed by: Dr. Adam Rudner Group: iGEM19_uOttawa (2019-10-16)
HF Phusion Polymerase
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 1430
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 1408
Design Notes
Coding sequence was codon-optimized for expression in E. coli and for the removal of illegal restriction sites.
Source
The coding sequence was derived from the genome of Escherichia phage T5. Reference for original sequence (prior to our adjustments): UniProtKB - P06229 (FEN_BPT5).