Coding
HF Phusion

Part:BBa_K3271027:Design

Designed by: Dr. Adam Rudner   Group: iGEM19_uOttawa   (2019-10-16)


HF Phusion Polymerase


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 1430
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 1408


Design Notes

Coding sequence was codon-optimized for expression in E. coli and for the removal of illegal restriction sites.


Source

The coding sequence was derived from the genome of Escherichia phage T5. Reference for original sequence (prior to our adjustments): UniProtKB - P06229 (FEN_BPT5).

References